Illuminate Your Research with Our Leading-Edge Lentiviral Reporters!

Find Your Reporter Lentivirus In Our Growing Catalog >>

Transcription Factor & Signaling Pathway

1. Signal Reception:

2. Signal Transduction:

3. Transcription Factor Activation:

4. DNA Binding and Transcriptional Regulation:

5. Gene Expression:

Example Pathway: NF-κB Signaling  >> NF-κB Reporter Lentivirus

Importance of Monitoring Transcriptional Activity

Understanding Cellular Responses:

Drug Discovery and Development:

Disease Mechanisms:

Transcription Factor (TF) Reporter Lentiviruses:

Why Choose Us?

Unrivaled Sensitivity – LipExoGen Reporter lentiviral particles are made using a novel vector platform based on the third generation system. The transcription factor’s response elements are arranged as DNA tandem repeats upstream of the minimal TATA promoter-driven reporter, and downstream of an optimized mini enhancer. When the signal pathway/TF is activated, the mini enhancer synergizes with TF binding to the response elements (up to 8 repeats in some products, depending on strength of reporter activation) to amplify expression of the fluorescent (GFP/RFP) or luciferase (Luc) reporter, with minimal enhancement of background. As a result, the reporter system provides a highly sensitive readout for signaling pathway or specific transcription factor activation in human and mouse cells.

Easily Establish Stable Reporter Cell Lines – The reporter lentiviral particles are ultra-purified and concentrated to high-titer by PEG purification and sucrose gradient centrifugation to allow for efficient transduction of difficult-to-transfect cells, including primary and/or freeze-thawed cells. Stable cell lines are easily generated through puromycin or blasticidin selection.

Discovery Made Easy – Signal pathway or specific transcription factor activity can be detected by fluorescence, making LipExoGen TF Reporter lentiviral particles more practical than traditional luciferase reporters and/or biochemical assays. Pathway/TF activation can be readout by flow cytometry or fluorescence microscopy in living cell cultures, providing more versatility in data acquisition for labs with different instruments.

Best Value – LipExoGen lentiviral particle products are made using optimized lentiviral vectors developed in-house, which allows us to provide the highest quality products while retaining competitive prices. These high-titer lentiviral particles feature a highly sensitive fluorescent reporter system which has been validated to read out the indicated transcription factor activity.

Completely Customizable – You can easily request a modified version of the lentiviral reporter, such as a dual-reporter or a different reporter not listed on our website, by contacting us.

Look Up Your Transcription Factor/Signaling Pathway in Our Growing Catalog   >> View All

Real-World Applications

Study Temporal Activation of Signaling Pathways

Make Stable Cell Lines & Detect Transcriptional Activity on Flow

Test Drugs & Monitor Transcriptional Activity In Vivo