JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Fish Probe

eFISH TP53/CEN 17 Dual Color Probe

SKU : BHR15100158

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

BioGenex Laboratories

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	FP062-10X
Gene Name	TP53/CEN 17
Fluorophore	Orange/Green
Chromosome	17
Intended Use	The BioGenex eFISH TP53/CEN 17 dual color probe is currently available for Researchuse only. eFISH TP53/CEN 17 dual color probe is designed to be used for the detection oftranslocations involving the TP53/CEN 17gene at 10q11.21 in formalin-fixed,paraffinembedded tissue or cells by fluorescence in situ hybridization (FISH).BioGenex eFISH TP53/CEN 17dual color probecomes in hybridization buffer. The probecontains orange-labeled polynucleotides (Orange: excitation at 547 nm and emission at572 nm, similar to rhodamine), which target the TP53 gene in 17p13.1, and green-labeledpolynucleotides (Green: excitation at 503 nm and emission at 528 nm, similar to FITC),which target alpha-satellite-sequences of the centromereof chromosome 17
Description	Fluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for thedetection of chromosomal aberrations, presence or absence of specific DNA sequence innative context. In this technique florescent probes bind to the target sequence of DNA inchromosome. High specificity and sensitivity coupled rapid and an accurate result hasproven role of FISH in both research and diagnosis of solid tumor and hematologicalmalignancies. As technique of cancer cytogenetics, FISH, can be used to identify geneticaberrations viz., deletions, amplification and translocation in tissue sections or withinindividual cells. FISH is also used for use in genetic counseling, medicine, and species identification. FISH can also be used to detect and localize specific RNA targets in cells,circulating tumor cells, and tissue samples1,2,3,4,5.In FISH procedure, fixed tissue sections are pretreated to expose target DNA or mRNAsequences. An appropriately labeled probe is hybridized to the exposed target DNA ormRNA sequences in the cells. Subsequent stringent washing steps remove any probe thatis non-specifically bound to the tissue section. Subsequently slides are mounted usingDAPI/antifade and can be visualized under fluorescence microscope using appropriate filterset.
Principle	In Situ hybridization (ISH) allows the detection and localization of definitive nucleic acidsequences directly within a cell or tissue. High specificity is ensured through the action ofannealing of fluorescence probe nucleic acid sequence to complementary target nucleicacid sequence. ISH techniques can be used to identify genetic aberrations like deletions,amplification, and translocationin tissue sections or within individual cells.
Storage	The BioGenex eFISH TP53/CEN 17dual color probe must be stored at 2-8°C protected from light and is stable through the expiry date printed on the label.

Reference	Datasheet SDS

No Description Available.

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHR15100158	FP062-10X	10 tests	$1152		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.