ECOS™ 21 (BL21)(DE3) Competent Cells 2*10^7 cfu/μg

Mfr.No.	FYE207-10VL, FYE207-40VL
Description	ECOS - E. Coli One-Step transformation. SOCE - SOC Eliminated This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Application	IPTG induced gene expression in E. coli Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors Subcloning
Efficiency	> 2 x 107 cfu/μg
Features	lack of Lon and Omp proteases allows high-level protein expression and accumulation Easy gene induction
Genotype	F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
Strain	BL21(DE3)
Species	E. coli

Product Format	Tube (100 μL) / 96-well plate (50 μL)
Storage	-70°C.
Package Size	Competent cells, pUC19 control plasmid

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHC17300003-1	FYE207-10VL	100 μl x 10 vials	$128		Add to Cart
BHC17300003-2	FYE207-40VL	100 μl x 40 vials	$389		Add to Cart