MegaFi™ Pro One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFi™ Pro Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. It provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR.
Template
RNA
Features
Enjoy simple, one-step RT-PCR reactions
over 2000X lower error rates due to our MegaFi™ Pro Fidelity DNA Polymerase (Cat. No. G886)
Extremely thermostable cDNA synthesis at 50-80°C due to our OneScript® Hot Reverse Transcriptase (Cat. No. G593)
Excellent protection against common ribonucleases due to our RNaseOFF Ribonuclease Ihibitor (Cat. No. G591)
Earn 2X the rewards points
Format
Kit
Quantity
RT-PCR Enzyme Mix
100 rxn (400 µl)
2X One-Step RT-PCR Buffer
2 x 1.25 ml
Reaction
Onestep RTPCR
Reference
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).