SensTaq HotStart DNA Polymerase is a new type of thermal stable DNA polymerase that is fused with a dsDNA binding subunit. This feature allows the DNA polymerase to stay binding on the template for longer, thus improving its sensitivity and processivity. The enzyme is also an antibody-based hotstart DNA polymerase which gives the enzyme a very good specificity. The proprietary antibody blocks polymerase activity at low temperatures. During the initial denaturation step, the antibody dissociates from the polymerase and restores enzyme activity. Therefore SensTaq is an ideal choice for detection assays and limited template situation such as single-cell target amplification. SensTaq HotStart DNA Polymerase has 5’ to 3’ exonuclease activity but lacks 3’ to 5’ proofreading activity, and it is able to amplify products up to 1.5kb in length containing a single base (A) overhang on the 3’ ends.
Literature
Quantity
250U
Reference
Chung, Get al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011).DOI: 10.1016/j.dnarep.2011.09.011.PubMed: 21968058. Application: PCR.
Bhagwat, Bet al. "An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation." J Genet Genomics 40 (5):261-270 (2013).DOI: 10.1016/j.jgg.2013.03.012.PubMed: 23706301. Application: PCR.
Jones, MRet al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012).DOI: 10.1007/s00438-012-0681-0.PubMed: 22350747. Application: PCR.
Chung, Get al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174-1182 (2011).DOI: 10.1016/j.dnarep.2011.09.011.PubMed: 21968058. Application: PCR.
Lee, Get al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59 (9):1347-1356 (2010).DOI: 10.1007/s00262-010-0864-7.PubMed: 20473495. Application: PCR.
Chaiyakul, Met al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus." J. Virol. 84 (21):11440-11447 (2010).DOI: 10.1128/JVI.01030-10.PubMed: 20810737. Application: PCR.
Lee, Get al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59 (9):1347-1356 (2010).DOI: 10.1007/s00262-010-0864-7.PubMed: 20473495. Application: PCR.
Ahn, CHet al. "Bacterial biofilm-community selection during autohydrogenotrophic reduction of nitrate and perchlorate in ion-exchange brine." Appl. Microbiol. Biotechnol. 81 (6):1169-1177 (2009).DOI: 10.1007/s00253-008-1797-3.PubMed: 19066883. Application: PCR.
Lee, Get al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker." Cancer Biomark 5 (4):177-188 (2009).DOI: 10.3233/CBM-2009-0102.PubMed: 19729827. Application: PCR.