Synthesize cDNA from only 0.1pg of RNA with abm’s EasyScript™ Reverse Transcriptase. Using strategic modifications, abm’s EasyScript™ Reverse Transcriptase lacks the intrinsic RNase H acitivity thereby drastically increases the yield and achievable synthesized cDNA length (up to 9kb). In addition, EasyScript™ contains a fidelity-enhancing subunit which ensures superior accuracy in reverse transcription reaction(s).
Template
RNA
Trade Marker
OneScript®
Buffer
Enzyme supplied with 5X RT buffer
Concentration
200 U/ul
Quantity
25 x 20 µl reactions
Reference
Bakir, M et al. "Relationship of plasma cell-free DNA level with mortality and prognosis in patients with Crimean-Congo hemorrhagic fever." J Med Virol : (2015). DOI: 10.1002/jmv.24446. PubMed: 26680021. Application: Reverse Transcription.
Baylink, D et al. "Systemic and local ex vivo gene therapy of the skeleton ." U.S. Patent No. 20, 160, 120, 944 : (2016).
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Dahiya, S et al. "Sequence analysis of the Toll-like receptor 2 gene of old world camels ." J. Adv. Res. : (2013). DOI: 10.1016/j.jare.2013.09.001. Application: Reverse Transcription.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Kim, BH et al. "Sophoraflavanone G induces apoptosis of human cancer cells by targeting upstream signals of STATs." Biochem. Pharmacol. 86(7):950-959 (2013). DOI: 10.1016/j.bcp.2013.08.009. Application: Reverse Transcription.
Kim, SJ et al. "Selective expression of transgene using hypoxia-inducible trans-splicing group I intron ribozyme.." J Biotechnol. Pt A:22-7 (2014). DOI: 10.1016/j.jbiotec.2014.10.001. PubMed: 25312327. Application: Reverse Transcription.
Lee, G et al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker." Cancer Biomark. 5(4):177-188 (2009). DOI: 10.3233/CBM-2009-0102. Application: Reverse Transcription.
Lee, G et al. "Carbohydrate-associated Immunodominant Epitope(s) of CA215." Immunol. Invest. 41(3):317-336 (2012). DOI: 10.3109/08820139.2011.633141. Application: Reverse Transcription.
Lee, G et al. "Growth inhibition of tumor cells in vitro by using monoclonal antibodies against gonadotropin-releasing hormone receptor." Cancer Immunol. Immunother 59(7):1011-1019 (2010). DOI: 10.1007/s00262-010-0823-3. Application: Reverse Transcription.
Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59:1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. Application: Reverse Transcription.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Lee, G et al. "Widespread expressions of immunoglobulin superfamily proteins in cancer cells." Cancer Immunol. Immunother. 61(1):89-99 (2012). DOI: 10.1007/s00262-011-1088-1. Application: Reverse Transcription.
Li, R et al. "Ultrafine particles from diesel vehicle emissions at different driving cycles induce differential vascular pro-inflammatory responses: implication of chemical components and NF-kappaB signaling." Part Fibre Toxicol 7:6 (2010). DOI: 10.1186/1743-8977-7-6. PubMed: 20307321. Application: Reverse Transcription.
Nagarajan, G et al. "Cloning and phylogenetic analysis of Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) from Indian dromedaries (Camelus dromedarius)." Comp. Immunol. Microbiol. Infect. Dis. 34(3):291-298 (2011). DOI: 10.1016/j.cimid.2011.01.005. Application: Reverse Transcription.
Nagarajan, G et al. "Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)." Res. Vet. Sci. 92(3):420-426 (2012). DOI: 10.1016/j.rvsc.2011.03.028. Application: Reverse Transcription.
Sun, T et al. "TGACG-Binding Factor 1 (TGA1) and TGA4 regulate salicylic acid and pipecolic acid biosynthesis by modulating the expression of Systemic Acquired Resistance Deficient 1 (SARD1) and Calmodulin-Binding Protein 60g (CBP60g)." New Phytol. 1:344-354 (2018). DOI: 10.1111/nph.14780. Application: Reverse transcription.
Tang, Y et al. "Similar Gene Regulation Patterns for Growth Inhibition of Cancer Cells by RP215 or Anti-Antigen Receptors." J. Cancer Sci. Ther. 5:200-208 (2013). DOI: 10.4172/1948-5956.1000207 . Application: Reverse Transcription.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.
Wang, Q et al. "Selenium Deficiency-Induced Apoptosis of Chick Embryonic Vascular Smooth Muscle Cells and Correlations with 25 Selenoproteins." Biol. Trace Elem. Res 2:407-415 (2017). DOI: 10.1007/s12011-016-0823-z . Application: Reverse transcription.
Zhang, Q et al. "ER quality control components UGGT and STT3a are required for activation of defense responses in bir1-1." PLoS One 10(3):e0120245 (2015). DOI: 10.1371/journal.pone.0120245. PubMed: 25775181. Application: Reverse Transcription.
Concentration
200 U/ul
Storage Condition
-20C
Shipping Condition
Dry Ice
Storage
Store all components at -20°C.
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