Extraction-free DNA amplification from whole blood samples with abm’s Bloodirect DNA Polymerase. With this unique enzyme, DNA can be amplified in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of abm’s Bloodirect DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing.
With abm’s Bloodirect DNA Polymerase, you can
• Eliminate lengthy DNA purification steps
• Achieve direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin
Template
DNA
Buffer
Enzyme supplied with 5X buffer
Concentration
1 U/μl
Format
Polymerase
Quantity
400 U (400 µl)
Reaction
PCR
Reference
Ahn, CH et al. "Bacterial biofilm-community selection during autohydrogenotrophic reduction of nitrate and perchlorate in ion-exchange brine." Appl. Microbiol. Biotechnol. 81 (6):1169-1177 (2009). DOI: 10.1007/s00253-008-1797-3. PubMed: 19066883. Application: PCR.
Bhagwat, B et al. "An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation." J Genet Genomics 40 (5):261-270 (2013). DOI: 10.1016/j.jgg.2013.03.012. PubMed: 23706301. Application: PCR.
Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus." J. Virol. 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. PubMed: 20810737. Application: PCR.
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Lee, G et al. "Cancer cell expressions of immunoglobulin heavy chains with unique carbohydrate-associated biomarker." Cancer Biomark 5 (4):177-188 (2009). DOI: 10.3233/CBM-2009-0102. PubMed: 19729827. Application: PCR.
Lee, G et al. "Inhibition of in vitro tumor cell growth by RP215 monoclonal antibody and antibodies raised against its anti-idiotype antibodies." Cancer Immunol. Immunother. 59 (9):1347-1356 (2010). DOI: 10.1007/s00262-010-0864-7. PubMed: 20473495. Application: PCR.