Extracting and preparing RNA templates directly from cultured cells to be reverse-transcribed into cDNA, with 5X All-In-One RT MasterMix. abm’s ExCellenCT Lysis Kit is an unique and effective alternative method to provide RNA templates that are extracted and processed directly from cultured cells in less than 15 minutes, unveiling reverse-transcription-ready RNA/cell lysate from cultured cells without the time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. RNA templates generated can then be directly used as template to be reverse-transcribed into cDNA using the 5X All-In-One RT MasterMix.
The 5X All-In-One RT MasterMix is a ready-to-use formulation of all the reagents necessary for first-strand cDNA synthesis, including abm’s proprietary EasyScriptTM Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a finely balanced ratio of Oligo (dT)s and Random Primers. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA directly from 10-105 cultured cells, suitable for a wide range of downstream applications.
This product is only intended for quick RNA extraction from cultured cells for two-steps RT-PCR or two-steps qRT-PCR gene expression analysis.
This product is not suitable for typical RNA/cDNA pool preparation.
Template
RNA
Format
MasterMix
Mix Concentration
5X
Quantity
25 Preps 100 x 20 µl reactions
Reaction
RT-PCR
Reference
Bradley, R et al. "Glucagon-like peptide-2 requires a full-complement of Bmi-1 for its proliferative effects in the murine small intestine." Endocrinology 7: (2016). DOI: http://dx.doi.org/10.1210/en.2016-1127 . Application: Reverse Transcription.
Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
Dai, X et al. "External Ca 2+ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells." Exp. Cell. Res. : (2016). DOI: http://dx.doi.org/10.1016/j.yexcr.2016.11.004. Application: Reverse Transcription.
Deng, H et al. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola." Process Biochemistry 10:1357-1362 (2016). DOI: http://dx.doi.org/10.1016/j.procbio.2016.07.013. Application: Reverse Transcription.
Deng, H et al. "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." Gene 1:67-72 (2016). DOI: http://dx.doi.org/10.1016/j.gene.2016.01.019. Application: Reverse Transcription.
Hanson, M et al. "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." BioMed Central 1:228 (2016). DOI: 10.1186/s12862-016-0805-y. Application: Reverse Transcription.
Jeon, H.J et al. "Comparison of the functional characterization between alkane monooxygenases for low-molecular-weight polyethylene biodegradation." Int. Biodeterior. Biodegrad. :202-208 (2016). DOI: http://dx.doi.org/10.1016/j.ibiod.2016.06.012. Application: Reverse Transcription.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Li, L et al. "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." Int. Immunopharmacol. :189-198 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.02.019. Application: Reverse Transcription.
Liu, F et al. "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING‐LIKE 1 (OsCOLE1) and OsCOLE1‐INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." New Phytologist 1:96-107 (2016). DOI: 10.1111/nph.14021. Application: Reverse Transcription.
Liu, J.C et al. "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." International Journal of Cardiology :388-393 (2016). DOI: http://dx.doi.org/10.1016/j.ijcard.2016.11.202. Application: Reverse Transcription.
Su, X et al. "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." Int. Immunopharmacol. :16-25 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.05.005. Application: Reverse Transcription.
Sun, M et al. "Hypoxia inducible factor‐1α‐induced IL‐33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." Clinical & Experimental Immunology : (2016). DOI: 10.1111/cei.12896. Application: Reverse Transcription.
Tuerdi, N et al. "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." Nanomed. Nanotechnol. Biol. Med. 7:1899-1907 (2016). DOI: http://dx.doi.org/10.1016/j.nano.2016.05.002. Application: Reverse Transcription.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.
Yang, S et al. "Natural Infection of Avian Hepatitis E Virus and Marek's Disease Virus in Brown Layer Chickens in China." Avian Diseases 3:698-704 (2016). Application: Reverse Transcription.
Zhou, H et al. "Antigen distribution of TMUV and GPV are coincident with the expression profiles of CD8α-positive cells and goose IFNγ." Scientific reports :25545 (2016). DOI: 10.1038/srep25545. Application: Reverse Transcription.
Zhou, H et al. "LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies." Frontiers in microbiology :166 (2016). DOI: 10.3389/fmicb.2016.00166. Application: Reverse Transcription.