From RNA template to cDNA in just 15 minutes completely hassle-free. With abm’s 5X All-In-One RT MasterMix, just add RNA and this optimized system will provide sensitive and reliable cDNA synthesis over a dynamic range of input RNA. The use of this MasterMix eliminates multiple component additions providing exceptional reproducibility and precision, in addition to offering the end-user ultimate convenience.
Key Features
• Reduction in handling errors with only 1 liquid transfer step
• Streamlined protocol suitable for high-throughput applications
• Simple set-up for any RNA template
• High reproducibility and excellent yield
Note - In case of no RT control, it is suggested to take a small aliquot of the 5X All-In-One RT MasterMix, and perform deactivation of the RT by incubating the small aliquot on heat block at 85°C for 5 -10 minutes prior to the reaction setup.
Template
RNA
Format
MasterMix
Mix Concentration
5X
Quantity
100 x 10 µl reactions
Reaction
RT-PCR
Reference
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Chung, G et al. "CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans." DNA Repair (Amst.) 10 (11):1174- 1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.
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Deng, H et al. "An efficient polyethylene glycol-mediated transformation system of lentiviral vector in Shiraia bambusicola." Process Biochemistry 10:1357-1362 (2016). DOI: http://dx.doi.org/10.1016/j.procbio.2016.07.013. Application: Reverse Transcription.
Deng, H et al. "Reference genes selection and relative expression analysis from Shiraia sp. SUPER-H168 productive of hypocrellin." Gene 1:67-72 (2016). DOI: http://dx.doi.org/10.1016/j.gene.2016.01.019. Application: Reverse Transcription.
Gagnon, J et al. "Chronic Exposure to TNFα Impairs Secretion of Glucagon-Like Peptide-1." Endocrinology 156(11):3950-60 (2015). DOI: 10.1210/en.2015-1361. PubMed: 26270730. Application: Reverse Transcription.
Gagnon, J et al. "Ghrelin is a novel regulator of GLP-1 secretion ." Diabetes 64(5):1513-21 (2015). DOI: 10.2337/db14-1176. PubMed: 25412624. Application: Reverse Transcription.
Gagnon, J et al. "Ghrelin is a Novel Regulator of Glucagon-like Peptide-1 Secretion.." Diabetes. : (2014). PubMed: 25412624. Application: Reverse Transcription.
Hanson, M et al. "Immune genes and divergent antimicrobial peptides in flies of the subgenus Drosophila." BioMed Central 1:228 (2016). DOI: 10.1186/s12862-016-0805-y. Application: Reverse Transcription.
Jeon, H.J et al. "Comparison of the functional characterization between alkane monooxygenases for low-molecular-weight polyethylene biodegradation." Int. Biodeterior. Biodegrad. :202-208 (2016). DOI: http://dx.doi.org/10.1016/j.ibiod.2016.06.012. Application: Reverse Transcription.
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans." Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer." Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Li, L et al. "Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes." Int. Immunopharmacol. :189-198 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.02.019. Application: Reverse Transcription.
Li, R et al. "Ultrafine particles from diesel vehicle emissions at different driving cycles induce differential vascular pro-inflammatory responses: implication of chemical components and NF-kappaB signaling." Part Fibre Toxicol 7:6 (2010). DOI: 10.1186/1743-8977-7-6. PubMed: 20307321. Application: Reverse Transcription.
Liu, F et al. "Interactions of Oryza sativa OsCONTINUOUS VASCULAR RING‐LIKE 1 (OsCOLE1) and OsCOLE1‐INTERACTING PROTEIN reveal a novel intracellular auxin transport mechanism." New Phytologist 1:96-107 (2016). DOI: 10.1111/nph.14021. Application: Reverse Transcription.
Liu, J.C et al. "Osthole inhibits the expressions of collagen I and III through Smad signaling pathway after treatment with TGF-β1 in mouse cardiac fibroblasts." International Journal of Cardiology :388-393 (2016). DOI: http://dx.doi.org/10.1016/j.ijcard.2016.11.202. Application: Reverse Transcription.
Su, X et al. "Adjuvant effect of docetaxel on HPV16 L2E6E7 fusion protein vaccine in a mouse model." Int. Immunopharmacol. :16-25 (2016). DOI: http://dx.doi.org/10.1016/j.intimp.2016.05.005. Application: Reverse Transcription.
Sun, M et al. "Hypoxia inducible factor‐1α‐induced IL‐33 expression in intestinal epithelia contributes to mucosal homeostasis in inflammatory bowel disease." Clinical & Experimental Immunology : (2016). DOI: 10.1111/cei.12896. Application: Reverse Transcription.
Tuerdi, N et al. "Preventive effects of simvastatin nanoliposome on isoproterenol-induced cardiac remodeling in mice." Nanomed. Nanotechnol. Biol. Med. 7:1899-1907 (2016). DOI: http://dx.doi.org/10.1016/j.nano.2016.05.002. Application: Reverse Transcription.
Unsoy, G et al. "Chitosan magnetic nanoparticles for pH responsive Bortezomib release in cancer therapy." Biomedicine & Pharmacotherapy 68(5):641-649 (2014). DOI: 10.1016/j.biopha.2014.04.003. PubMed: 24880680. Application: Reverse Transcription.
Yang, S et al. "Natural Infection of Avian Hepatitis E Virus and Marek's Disease Virus in Brown Layer Chickens in China." Avian Diseases 3:698-704 (2016). Application: Reverse Transcription.
Zhang, Chi et al. "Primate-specific miR-603 is implicated in the risk and pathogenesis of Alzheimer's disease." Aging (Albany NY) 2:272-290 (2016). DOI: 10.18632/aging.100887. Application: RT.
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