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Home / Enzymes, Inhibitors & Substrates

NheI

SKU : BHZ10900073

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Applied Biological Materials (abm) Inc.

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Mfr.No.	E064
Description	Restriction enzyme digestion of double-stranded DNA
Components	NheI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes	Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by some combinations of overlapping
Concentration	10000 U/ml
Heat Inactivation	80°C for 20 min
Quantity	5000 U (500 µl)
Recognition Site	Sequence G^CTAGC
Source	An E. coli strain that carries the cloned and modified NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999). The NheI enzyme recognizes and cuts the sequence G^CTAGC.

Storage	-20°C
Shipping Condition	Ice Packs
Storage Condition	-20C
Buffer	Storage Buffer: 10mM Tris pH (7.5), 250mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol. Reaction Buffer: Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100%
Format	Endonuclease
Concentration	10000 U/ml

QC	Test Study- Restriction Enzyme Stability
Protocol	NheI E064 Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHZ10900073	E064	500µl	$154.44		Add to Cart

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