Restriction enzyme digestion of double-stranded DNA
Components
MfeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping
Concentration
20000 U/ml
Heat Inactivation
No
Quantity
10000 U (500 µl)
Recognition Site
Sequence C^AATTG
Source
An E.coli strain that carries the modified MfeI gene from Mycoplasma fermentas (N.F. Halden). The MfeI enzyme recognizes and cuts the sequence C^AATTG.