Restriction enzyme digestion of double-stranded DNA
Components
EcoRV enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer
Notes
Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping
Concentration
20000 U/ml
Heat Inactivation
65°C for 20 min
Quantity
10000 U (500 µl)
Recognition Site
Sequence GAT^ATC
Source
An E.coli strain that carries the modified EcoRV gene from the plasmid J62 pLG74 (L.l. Glatman). The EcoRV enzyme recognizes and cuts the sequence GAT^ATC.