Citrate Assay kit

Mfr.No.	CIT100
Description	Citrate is an important biomolecule and popular additive in pharmaceutical products. The Citrate Assay Kit is for measurement of sub-millimolar to low millimolar concentrations of citrate. The assay is based on increase of the optical density at 490 nm (OD490) of the kit reagent upon binding to citrate. The assay is compatible with Tris-HCl buffers. It is not compatible with phosphate buffers, polycarboxylic acids or other poly acids. The Citrate Assay kit (catalog number CIT100) includes 1.5 ml of Reagent A, 1.5 ml of 10 x Reagent B and 0.2 ml of 100 mM ammonium citrate. It is for 100 assays using 96-well plates. Cuvettes may also be used for measurements.

Product Manual	Datasheet
Protocols Description	PROTOCOL Standard curve 1. Prepare 150 µl of citrate solution in water or 10 mM Tris-HCl, pH 7, at a series of concentrations ranging from 5 mM to zero. 2. Add 15 µl of Reagent A. 3. Prepare 1 x Reagent B by dilution of 10 x Reagent B 10 -fold with ethanol. Each assay well uses 150 µl of 1 x Reagent B. 4. Add 150 µl of 1 x Reagent B and mix the solutions evenly by pipetting up-and-down a few times. 5. Read the optical density (OD₄₉₀) in 10 min. Data Analysis Plot the OD₄₉₀ values and the citrate concentrations [Citrate] to generate the linear standard curve. OD₄₉₀ = a [Citrate] + b Where the OD₄₉₀values are from experimental data, the a and b values are from the linear fitting between the OD₄₉₀ values and the citrate concentrations. UNKNOWN SAMPLES Follow the same procedure to measure the A650values from the unknown samples. Calculate the citrate concentrations in the unknown samples using the OD₄₉₀ values from the unknown samples and the a and b values from the standard curve. [Citrate] = (OD₄₉₀– b) / a

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300245	CIT100	100 assays	$211.16		Add to Cart