MycoFold rHu PDGF-BB

MycoFold™ Recombinant Human Platelet-Derived Growth Factor Subunit B (rHu PDGF-BB) is a growth factor that plays an essential role in regulating embryonic development, cell proliferation, migration, survival, and chemotaxis.  This protein is a potent mitogen that stimulates the proliferation of cells of mesenchymal origin, such as fibroblasts, smooth muscle cells, and pericytes, and promotes cell survival by activating anti-apoptotic signaling pathways.  PDFG-BB has the broadest receptor binding profile among the PDGF isoforms, allowing it to activate all three receptor dimeric combinations (PDGFRaa, PDGFRab, and PDGFRbb).  Once the receptor is activated, numerous intracellular cascades are triggered, including PI3K/AKT/mTOR, MAPK, PLCg, JAK/STAT, RHO GTPase, CRKL/C3G/RAP1, and others, which lead to cell proliferation, migration, and survival. PDGF-BB binding leads to upregulation of transcription factors and genes, including (i) cMyc, c-Fos, c-Jun (proliferation), (ii) NF-kB (survival and inflammation), (iii) HIFa (angiogenesis), and (iv) Cyclin D1 (cell cycle progression).

Protein is an antiparallel, disulfide-linked homodimer (12.3 kDa) expressed via extracellular secretion of K. phaffii engineered yeast.  Chaperone-guided folding and engineered secretion pathways generate native tertiary 3D structures, thereby optimizing thermostability.  Priced for best-in-class bioactivity value (IUs/mg/$) to maximize research budgets.

Key Research Applications

• Differentiation of oligodendrocyte precursors and mesenchymal stem cells (MSCs)
• Blood-brain barrier (BBB) integrity due to pericyte loss due to impaired PDGF-BB signaling
• Cell proliferation and control stimulus of fibroblasts, smooth muscle cells, and pericytes
• Osteoblast and chondrocyte proliferation and differentiation
• Tumor stroma formation and the role of cancer-associated fibroblasts
• Autocrine and paracrine PDGF-BB signaling in tumor progression
• Tissue repair and molecular mechanisms of granulation tissue formation in wound healing and regenerative medicine.
• Biomaterial research incorporating hydrogels and or scaffolds to promote tissue regeneration.