Purified recombinant fragment of human MAPK expressed in E. Coli.
Purification
Unpurified ascites.
Isotype
IgG
Tested Application
ELISA, WB, IHC, FCM
Uniprotkb
P27361 (UniProt, ExPASy) P28482 (UniProt, ExPASy)
Gene Id
5594
Availability
Shipped within 5-10 working days.
Target
p44/42 MAPK (Erk1/2)
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reactivity
Human, Mouse
Molecular Weight
42 kDa
Note
This product is for research use only.
Clonality
monoclonal
Buffer
Ascitic fluid containing 0.03% sodium azide.
Recommended Dilution
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Description
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.
Immunohistochemical analysis of paraffin-embedded human Liver tissues using anti-BHMT antibody.
Flow cytometric analysis of Jurkat cells using p44/42 MAPK antibody (green) and negative control (purple).