Loading ....
Unveiling DENARASE® High Salt: A Pioneering Solution for Biopharmaceutical Manufacturing
Published On 03/14/2024 5:33 PM
In the dynamic realm of biopharmaceutical manufacturing, the quest for enhanced efficiency and precision in DNA removal processes is relentless. The introduction of DENARASE® High Salt by c-LEcta GmbH marks a significant leap forward, offering a specialized endonuclease engineered for high salt tolerance, thus broadening the spectrum of its applicability in various bioprocessing contexts.
Product Overview
DENARASE® High Salt is a recombinant Serratia marcescens endonuclease, meticulously engineered to maintain robust activity across a wide range of salt concentrations and pH levels. This adaptability makes it an ideal candidate for DNA removal in biomanufacturing processes, especially where high monovalent cation concentrations are beneficial or necessary.
Key Applications
This innovative enzyme finds its utility in numerous applications, notably:
- Viral vector production for cell and gene therapies.
- Manufacturing of viral vaccines.
- Reduction of viscosity in lysates.
- Sample preparation in electrophoresis and chromatography.
Technical Specifications
The product is available in various units, ranging from 25 kU to 5000 kU, catering to diverse research and development needs. Each batch is rigorously tested to ensure a purity level of ≥99% and an activity of >250 U/µl, adhering to stringent quality standards.
Operational Flexibility
DENARASE® High Salt's ability to operate effectively under elevated salt conditions (200-500 mM) without compromising its nucleic acid cleavage efficiency offers substantial benefits in terms of process flexibility and efficiency. Its compatibility with various pH levels (7.4 – 8.0) further underscores its versatility in different bioprocessing environments.
Compliance and Quality Assurance
Produced under the ISO 9001 standard, DENARASE® High Salt is designed for R&D applications, with a GMP-grade version slated for future release. Its production eschews the use of antibiotics, Triton X-100, and materials of animal origin, ensuring compliance with stringent industry standards.
Innovative Engineering
The product's engineering prowess is evident in its enhanced salt tolerance, achieved through precise amino acid substitutions. This has not only expanded its functional range but also retained its specificity for nucleic acids, ensuring efficient cleavage across a broader operational spectrum.
Conclusion
DENARASE® High Salt represents a significant advancement in the field of bioprocessing, offering unparalleled versatility and efficiency in DNA removal. Its introduction is poised to set new benchmarks in the industry, reinforcing c-LEcta GmbH's commitment to innovation and quality in biomanufacturing solutions.
This entry was posted in
Product Literature
,Application and Technique Notes
