Anti-Zebrafish SHP2/PTPN11 Antibody Picoband®

Mfr.No.	AZQ7ZW17
Background	PTPN11(Tyrosine-protein phosphatase non-receptor type 11), also known as protein-tyrosine phosphatase 1D(PTP-1D), protein-tyrosine phosphatase 2C(PTP-2C), TYROSINE PHOSPHATASE SHP2(SHP2), BPTP3, SH-PTP2, SHP-2, SH-PTP3, is an enzyme that in humans is encoded by the PTPN11 gene. PTPN11 is a member of the protein tyrosine phosphatase(PTP) family. The open reading frame consists of 1,779 nucleotides potentially encoding a protein of 593 amino acids with a predicted molecular mass of 68 kD. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish SHP2/PTPN11 Antibody Picoband® catalog # AZQ7ZW17. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish SHP2/PTPN11 recombinant protein (Position: T2-K99).
Molecular Weight	68 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Tyrosine-protein phosphatase non-receptor type 11
Gene Name	PTPN11
Weight	68 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB
Description	AZQ7ZW17 is guaranteed for WB Boster Guarantee

Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (AZQ7ZW17). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2/PTPN11 antigen affinity purified polyclonal antibody (AZQ7ZW17) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (C

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011452-1	AZQ7ZW17	Unconjugated	100 ug	$470	Add to Cart
BHA21011452-2	AZQ7ZW17-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011452-3	AZQ7ZW17-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011452-4	AZQ7ZW17-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011452-5	AZQ7ZW17-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011452-6	AZQ7ZW17-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011452-7	AZQ7ZW17-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011452-8	AZQ7ZW17-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011452-9	AZQ7ZW17-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011452-10	AZQ7ZW17-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011452-11	AZQ7ZW17-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011452-12	AZQ7ZW17-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011452-13	AZQ7ZW17-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish SHP2/PTPN11 Antibody PicobandÂ®

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