Anti-Zebrafish MYD88 Antibody Picoband®

Mfr.No.	AZQ5XJ85
Background	MYD88(MYELOID DIFFERENTIATION PRIMARY RESPONSE GENE 88), is a protein that, in humans, is encoded by the MYD88 gene. MyD88 is a key downstream adapter for most Toll-like receptors (TLRs) and interleukin-1 receptors (IL1Rs). And it is mapped on 3p22.2. MYD88 encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. Overexpression of MYD88 caused an increase in the level of transcription from the interleukin-8 promoter. The C-terminal domain of MYD88 has significant sequence similarity to the cytoplasmic domain of IL1RAP. Inhibiting the IL1R-MYD88 pathway in vivo could block the damage from acute inflammation that occurs in response to sterile cell death, and do so in a way that might not compromise tissue repair or host defense against pathogens.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish MYD88 Antibody Picoband® catalog #AZQ5XJ85. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish MYD88 recombinant protein (Position: M1-K266)
Molecular Weight	33 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish liver tissue, zebrafish colon tissue, zebrafish thymus tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Myeloid differentiation primary response protein MyD88
Gene Name	MYD88
Weight	33 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ5XJ85 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). MYD88 was detected in a paraffin-embedded section of zebrafish thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MYD88 Antibody (AZQ5XJ85) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates.Lane 2: whole female zebrafish tissue lysates.Lane 3: whole male zebrafish tissue lysates.Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (AZQ5XJ85) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-20

IHC analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). MYD88 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MYD88 Antibody (AZQ5XJ85) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). MYD88 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MYD88 Antibody (AZQ5XJ85) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Have you used this product?

Rate it now..!!

Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011349-1	AZQ5XJ85	Unconjugated	100 ug	$470	Add to Cart
BHA21011349-2	AZQ5XJ85-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011349-3	AZQ5XJ85-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011349-4	AZQ5XJ85-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011349-5	AZQ5XJ85-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011349-6	AZQ5XJ85-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011349-7	AZQ5XJ85-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011349-8	AZQ5XJ85-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011349-9	AZQ5XJ85-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011349-10	AZQ5XJ85-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011349-11	AZQ5XJ85-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011349-12	AZQ5XJ85-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish MYD88 Antibody PicobandÂ®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Anti-Zebrafish MYD88 Antibody PicobandÂ®

Rate it now..!!