Anti-Zebrafish MPEG1 Antibody

Mfr.No.	AZQ7SXE0
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish MPEG1 Antibody catalog #AZQ7SXE0. Tested in IHC applications. This antibody reacts with Zebrafish.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish MPEG1 recombinant protein (Position: Q68-S718)
Positive Control	IHC: zebrafish spinal cord tissue, zebrafish testis tissue, zebrafish pancreas tissue, zebrafish liver tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Macrophage-expressed gene 1 protein
Gene Name	MPEG1
Weight	78.39kDa

Dilutions	Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC
Description	AZQ7SXE0 is guaranteed for IHC Boster Guarantee

IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. +1 MPEG1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MPEG1 Antibody (AZQ7SX

IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011356-1	AZQ7SXE0	Unconjugated	100 ug	$470	Add to Cart
BHA21011356-2	AZQ7SXE0-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011356-3	AZQ7SXE0-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011356-4	AZQ7SXE0-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011356-5	AZQ7SXE0-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011356-6	AZQ7SXE0-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011356-7	AZQ7SXE0-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011356-8	AZQ7SXE0-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011356-9	AZQ7SXE0-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011356-10	AZQ7SXE0-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011356-11	AZQ7SXE0-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011356-12	AZQ7SXE0-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish MPEG1 Antibody

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