Anti-Zebrafish MMP9 Antibody

Mfr.No.	AZQ7T317
Background	Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish MMP9 Antibody catalog #AZQ7T317. Tested in IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish MMP9 recombinant protein (Position: D33-L680)
Positive Control	IHC: zebrafish colon tissue, zebrafish muscle tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Matrix metalloproteinase-9
Gene Name	MMP9
Weight	78.458kDa

Dilutions	Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC
Description	AZQ7T317 is guaranteed for IHC Boster Guarantee

IHC analysis of MMP9 using anti-MMP9 antibody (AZQ7T317). MMP9 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MMP9 Antibody (AZQ7T317) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of MMP9 using anti-MMP9 antibody (AZQ7T317). MMP9 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MMP9 Antibody (AZQ7T317) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of MMP9 using anti-MMP9 antibody (AZQ7T317). MMP9 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-MMP9 Antibody (AZQ7T317) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Have you used this product?

Rate it now..!!

Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011389-1	AZQ7T317	Unconjugated	100 ug	$470	Add to Cart
BHA21011389-2	AZQ7T317-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011389-3	AZQ7T317-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011389-4	AZQ7T317-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011389-5	AZQ7T317-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011389-6	AZQ7T317-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011389-7	AZQ7T317-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011389-8	AZQ7T317-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011389-9	AZQ7T317-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011389-10	AZQ7T317-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011389-11	AZQ7T317-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011389-12	AZQ7T317-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish MMP9 Antibody

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Anti-Zebrafish MMP9 Antibody

Rate it now..!!