Anti-Zebrafish IDH3B Antibody

Mfr.No.	AZA0A2R8QPC9
Background	Isocitrate dehydrogenase (NAD) subunit beta, mitochondrial is an enzyme that in humans is encoded by the IDH3B gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alteatively spliced transcript variants encoding different isoforms have been described for this gene.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish IDH3B Antibody catalog #AZA0A2R8QPC9. Tested in IHC applications. This antibody reacts with Zebrafish.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish IDH3B recombinant protein (Position: T33-D363)
Positive Control	IHC: zebrafish liver tissue, zebrafish pancreas tissue, zebrafish heart tissue, zebrafish muscle tissue, zebrafish spinal cord tissue, zebrafish cerebellum tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Isocitrate dehydrogenase (NAD) subunit beta, mitochondrial
Gene Name	IDH3B
Weight	42.184kDa

Dilutions	Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC
Description	AZA0A2R8QPC9 is guaranteed for IHC Boster Guarantee

IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. +3 IDH3B was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-IDH3B An

IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011330-1	AZA0A2R8QPC9	Unconjugated	100 ug	$470	Add to Cart
BHA21011330-2	AZA0A2R8QPC9-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011330-3	AZA0A2R8QPC9-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011330-4	AZA0A2R8QPC9-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011330-5	AZA0A2R8QPC9-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011330-6	AZA0A2R8QPC9-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011330-7	AZA0A2R8QPC9-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011330-8	AZA0A2R8QPC9-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011330-9	AZA0A2R8QPC9-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011330-10	AZA0A2R8QPC9-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011330-11	AZA0A2R8QPC9-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011330-12	AZA0A2R8QPC9-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011330-13	AZA0A2R8QPC9-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish IDH3B Antibody

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