Anti-Zebrafish GNAQ Antibody Picoband®

Mfr.No.	AZB8JLJ7
Background	Guanine nucleotide-binding protein G(q) subunit alpha is a protein that in humans is encoded by the GNAQ gene. Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GDP for GTP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta. Mutations in this gene have been found associated to cases of Sturge-Weber syndrome and port-wine stains.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish GNAQ Antibody Picoband® catalog # AZB8JLJ7. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish GNAQ recombinant protein (Position: K102-D138).
Molecular Weight	42 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish brain tissue, zebrafish esophagus tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Guanine nucleotide-binding protein G(q) subunit alpha
Gene Name	GNAQ
Weight	42 kDa

Dilutions	Western blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZB8JLJ7 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of GNAQ using anti-GNAQ antibody (AZB8JLJ7). GNAQ was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GNAQ Antibody (AZB8JLJ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of GNAQ using anti-GNAQ antibody (AZB8JLJ7). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAQ antigen affinity purified polyclonal antibody (AZB8JLJ7) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200)

IHC analysis of GNAQ using anti-GNAQ antibody (AZB8JLJ7). GNAQ was detected in a paraffin-embedded section of zebrafish esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GNAQ Antibody (AZB8JLJ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011460-1	AZB8JLJ7	Unconjugated	100 ug	$470	Add to Cart
BHA21011460-2	AZB8JLJ7-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011460-3	AZB8JLJ7-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011460-4	AZB8JLJ7-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011460-5	AZB8JLJ7-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011460-6	AZB8JLJ7-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011460-7	AZB8JLJ7-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011460-8	AZB8JLJ7-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011460-9	AZB8JLJ7-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011460-10	AZB8JLJ7-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011460-11	AZB8JLJ7-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011460-12	AZB8JLJ7-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011460-13	AZB8JLJ7-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish GNAQ Antibody PicobandÂ®

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