Anti-Zebrafish GBF1 Antibody Picoband®

Mfr.No.	AZA0A286Y9X1
Background	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the GBF1 gene. This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish-GBF1-Antibody Picoband® catalog # AZA0A286Y9X1. Tested in WB, IHC, IF applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish GBF1 recombinant protein (Position: E110-K1605).
Molecular Weight	207 kDa
Positive Control	IF: zebrafish embryo tissue WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish brain tissue, zebrafish heart tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Gene Name	GBF1
Weight	207 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish Immunofluorescence, 5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IF, IHC, WB
Description	AZA0A286Y9X1 is guaranteed for IF, IHC, WB Boster Guarantee

IHC analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). GBF1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GBF1 Antibody (AZA0A286Y9X1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. +1 GBF1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ?g/mL rabbit anti-GBF1 Antibody (AZA

IHC analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). GBF1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GBF1 Antibody (AZA0A286Y9X1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBF1 antigen affinity purified polyclonal antibody (AZA0A286Y9X1) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR119

IHC analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). GBF1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GBF1 Antibody (AZA0A286Y9X1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011424-1	AZA0A286Y9X1	Unconjugated	100 ug	$470	Add to Cart
BHA21011424-2	AZA0A286Y9X1-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011424-3	AZA0A286Y9X1-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011424-4	AZA0A286Y9X1-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011424-5	AZA0A286Y9X1-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011424-6	AZA0A286Y9X1-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011424-7	AZA0A286Y9X1-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011424-8	AZA0A286Y9X1-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011424-9	AZA0A286Y9X1-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011424-10	AZA0A286Y9X1-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011424-11	AZA0A286Y9X1-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011424-12	AZA0A286Y9X1-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011424-13	AZA0A286Y9X1-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish GBF1 Antibody PicobandÂ®

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