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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Zebrafish ASH2L Antibody

SKU : BHA21011466

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Mfr.No.	AZA0A8M2B2E1
Background	Set1/Ash2 histone methyltransferase complex subunit ASH2 is an enzyme that in humans is encoded by the ASH2L gene. The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator. While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4. These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity. MLL1 and MLL2 complexes contain the additional protein subunit, menin. Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4. MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish ASH2L Antibody catalog # AZA0A8M2B2E1. Tested in IHC, IF applications. This antibody reacts with Zebrafish.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish ASH2L recombinant protein (Position: N95-G584).
Positive Control	IF: zebrafish embryo tissue, zebrafish embryo tissue, zebrafish embryo tissue IHC: zebrafish testis tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Set1/Ash2 histone methyltransferase complex subunit ASH2
Gene Name	ASH2L
Weight	68.723kDa

Dilutions	Immunohistochemistry, 2-5 ?g/ml, Zebrafish Immunofluorescence, 2 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IF, IHC
Description	AZA0A8M2B2E1 is guaranteed for IF, IHC Boster Guarantee

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21011466-1	AZA0A8M2B2E1	Unconjugated	100 ug	$470		Add to Cart
BHA21011466-2	AZA0A8M2B2E1-10ug	Unconjugated	10ug sample (liquid)	$99		Add to Cart
BHA21011466-3	AZA0A8M2B2E1-Biotin	Biotin conjugated	100 ug	$670		Add to Cart
BHA21011466-4	AZA0A8M2B2E1-Cy3	Cy3 conjugated	100 ug	$670		Add to Cart
BHA21011466-5	AZA0A8M2B2E1-Dylight488	Dylight488 conjugated	100 ug	$670		Add to Cart
BHA21011466-6	AZA0A8M2B2E1-Dylight550	Dylight550 conjugated	100 ug	$670		Add to Cart
BHA21011466-7	AZA0A8M2B2E1-Dylight594	Dylight594 conjugated	100 ug	$670		Add to Cart
BHA21011466-8	AZA0A8M2B2E1-FITC	FITC conjugated	100 ug	$670		Add to Cart
BHA21011466-9	AZA0A8M2B2E1-HRP	HRP conjugated	100 ug	$670		Add to Cart
BHA21011466-10	AZA0A8M2B2E1-APC	APC conjugated	100 ug	$770		Add to Cart
BHA21011466-11	AZA0A8M2B2E1-PE	PE conjugated	100 ug	$770		Add to Cart
BHA21011466-12	AZA0A8M2B2E1-iFluor647	iFluor647 conjugated	100 ug	$770		Add to Cart
BHA21011466-13	AZA0A8M2B2E1-carrier-free	Carrier Free	100 ug	$470		Add to Cart

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