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Home / VECTOR &VIRUS / Lentivirus

PD1-CD28/NFAT Reporter Lentivirus

SKU : BHV19400183

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Mfr.No.	TRV-0002, TRV-0002-1S, TRV-0002-2S, TRV-0002-3S, TRV-0002-4S, TRV-0002-5S, TRV-0002-6S, TRV-0002-7S, TRV-0002-8S
Description	The PD1-CD28/NFAT Immunotherapy Reporter is designed to measure the transcriptional activation of the NFAT pathway in response to modulation of PD1/CD28 expressed on the cell surface. The all-in-one reporter construct allows the user to easily generate stable reporter cell lines which constitutively express a cell surface receptor/ligand of interest (PD1 and CD28) and drug selection marker (Puro or BSD), and conditionally express a fluorescent (GFP or RFP) and secreted Gaussia luciferase (GLuc) dual reporter in response to transcriptional activation of the specific signaling pathway (NFAT). Constructs with constitutively expressed fluorescent proteins instead of a drug selection marker are also available for users who prefer to isolate transduced cells by FACS. This can be desirable, for example, in cases where the cells are difficult to culture or need to be rapidly transplanted in vivo. The PD1-CD28/NFAT Reporter provides a useful means for the user to assay T cell activity related to PD1/CD28 signaling in the cells of their choosing. This product is supplied as high-titer lentiviral particles, which have been purified by PEG precipitation and sucrose gradient centrifugation. The lentiviral particles are VSV-G pseudotyped and are ideal for difficult-to-transfect cells including primary or thawed cells. Product Format: High-titer Lentiviral Particles, third generation, VSV-G pseudotyped Bulk solution: Serum-free RPMI-1640, frozen solution Unit of measure: One vial cDNA Expressed: Human PD1 and CD28, separated by 2A (C-terminal Flag)
Promoter	EF1α
Titer	1-2×10^6 TU/vial, depending on the variant
Reporter	RFP, RFP-P2A-GLuc, GFP-P2A-GLuc, GFP
Selection	Blasticidin, RFP, GFP, Puromycin
Applications Range	Immune checkpoint inhibitor assay, T cell activation assay. The product can be used to generate stable cell lines that read out PD1/CD28 signaling through downstream activity of NFAT, for example in stable T cell lines. The fluorescent and luminescent dual reporter provides flexibility in the readout approach. GLuc is secreted, allowing the user to detect it in various biological media. Generally, the product is useful for analyzing T cell activity as a function of PD1/CD28 stimulation/inhibition.
Virus Type	Immunotherapy Reporter Lentivirus
Storage	-80oC

Reference Paper	Datasheet

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Product Name	Your price	Quantity
BHV19400183-1	TRV-0002-1S	2x10^6	GFP (constitutively expressed)	RFP	PD1-CD28-GFP/NFAT-RFP	$895		Add to Cart
BHV19400183-2	TRV-0002-2S	2x10^6	RFP (constitutively expressed)	GFP	PD1-CD28-RFP/NFAT-GFP	$895		Add to Cart
BHV19400183-3	TRV-0002-3S	2x10^6	GFP (constitutively expressed)	GLuc	PD1-CD28-GFP/NFAT-GLuc	$895		Add to Cart
BHV19400183-4	TRV-0002-4S	2x10^6	RFP (constitutively expressed)	GLuc	PD1-CD28-RFP/NFAT-GLuc	$895		Add to Cart
BHV19400183-5	TRV-0002-5S	1x10^6	Puromycin	GFP-P2A-GLuc	PD1-CD28-BSD/NFAT-GFP-GLuc	$895		Add to Cart
BHV19400183-6	TRV-0002-6S	1x10^6	Blasticidin	GFP-P2A-GLuc	PD1-CD28-BSD/NFAT-GFP-GLuc	$895		Add to Cart
BHV19400183-7	TRV-0002-7S	1x10^6	Puromycin	RFP-P2A-GLuc	PD1-CD28-Puro/NFAT-RFP-GLuc	$895		Add to Cart
BHV19400183-8	TRV-0002-8S	1x10^6	Blasticidin	RFP-P2A-GLuc	PD1-CD28-BSD/NFAT-RFP-GLuc	$895		Add to Cart

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