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Home / VECTOR &VIRUS / AAV Plasmids

pAAVdual-K14-EGFP

SKU : BHV17900070

This plasmid offers a relative flexible, simple, low cost, but high consistency and high efficiency for production of AAV virus with different AAV serotypes.

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AAVnerGene Inc

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Description
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Mfr.No.	SL035001-10ug
Description	pAAVdual-K14-EGFP is used to produce AAV-K14-EGFP virus using AAVnerGene s novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a K14 promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-K14-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions. The K14 promoter is an epithelial-specific regulatory element that drives gene expression in the basal layer of the epidermis and other stratified epithelia. It is a critical tool in skin and epithelial research, gene therapy for skin disorders, and the creation of transgenic models to study skin development and disease. Its specificity to basal epithelial cells makes it invaluable for targeting gene expression in the skin and related tissues, particularly in the context of studying and treating epithelial diseases.
Promoter	K14
Insert Gene	EGFP
AAV Cassette	AAV-K14-EGFP
Bacterial Resistance	Kana
Genome Size	2167 bp
Genome Type	ssAAV
ITRs	L-ITR (119 bp) + R-ITR (130 bp)
Plasmid Size	10641 bp
Production Systems	pAAVdual
Plasmid Backbone	pAAVdual
Storage	-20°C for long term (1 year)
Shipping	Dry ice
Notes	Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone. AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin). AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.

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SKU	Supplier No.	Size	Your price	Quantity
BHV17900070	SL035001-10ug	10 ug	$999		Add to Cart
BHV17900070	SL035001-10ug	100 ug	$1199		Add to Cart
BHV17900070	SL035001-10ug	1 mg	$1399		Add to Cart
BHV17900070	SL035001-10ug	10 mg	$1899		Add to Cart

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