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Home / VECTOR &VIRUS / Lentivirus

NF-kappaB Reporter Lentivirus

SKU : BHV19400002

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Mfr.No.	LTV-0002, LTV-0002-1S, LTV-0002-2S, LTV-0002-3S, LTV-0002-4S, LTV-0002-5S, LTV-0002-6S, LTV-0002-7S, LTV-0002-8S, LTV-0002-9S, LTV-0002-10S, LTV-0002-11S, LTV-0002-12S, LTV-0002-13S, LTV-0002-14S, LTV-0002-15S, LTV-0002-16S, LTV-0002-4SIC
Description	NFkappaB Reporter Lentivirus (Pan): High-quality lentiviral reporter system that provides a sensitive fluorescent (GFP/RFP) or luminescent (Luc) readout for human/mouse nuclear factor kappa B (NF-kB, NF-kappa B) activity in mammalian cells. The construct contains a minimal TATA promoter and tandem repeats of NFkappaB DNA-binding elements that drive expression of the reporter when bound to NFkappaB. An optimized mini-enhancer amplifies NFkappaB-dependent reporter expression resulting in a strong fluorescent/luminescent signal that can be used to readout NFkappaB pathway activity. An EF1a-driven selection marker (Puro/BSD) allows the user to easily create stable cell lines. This product is supplied as high-titer lentiviral particles which are VSV-G pseudotyped and ready-to-transduce a broad range of cell types. The lentiviral particles are purified by PEG precipitation and sucrose gradient centrifugation, serum-free, and ideal for studying NFkappaB signaling pathway activity in difficult-to-transfect cells including primary and/or thawed cells.
Serotype	VSV-G
Reporter	SEAP, Firefly Luc, RFP, GFP-P2A-FLuc, RFP-P2A-FLuc, BFP, Renilla Luc, GFP
Response Element	NF-kappaB response element
Selection	Blasticidin, RFP, GFP, Puromycin
Signaling pathway	NF-kappaB signaling pathway
Species	Human/mouse
Applications Range	Monitor NFkappaB pathway activation. Screen for activators or inhibitors of the NFkappaB pathway with fluorescence or luciferase. The fluorescent reporters enable convenient readout using flow cytometry, fluorescence microscopy, etc.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Your price	Quantity
BHV19400002-1	LTV-0002-1S	5x10^6	Puromycin	GFP	$595		Add to Cart
BHV19400002-2	LTV-0002-2S	5x10^6	Blasticidin	RFP	$595		Add to Cart
BHV19400002-3	LTV-0002-3S	2x10^6	Puromycin	Firefly Luc	$595		Add to Cart
BHV19400002-4	LTV-0002-4S	2x10^6	Blasticidin	Firefly Luc	$595		Add to Cart
BHV19400002-5	LTV-0002-5S	5x10^6	Blasticidin	GFP	$595		Add to Cart
BHV19400002-6	LTV-0002-6S	5x10^6	Puromycin	RFP	$595		Add to Cart
BHV19400002-7	LTV-0002-7S	2x10^6	Puromycin	SEAP	$595		Add to Cart
BHV19400002-8	LTV-0002-8S	2x10^6	Blasticidin	SEAP	$595		Add to Cart
BHV19400002-9	LTV-0002-9S	1x10^6	Puromycin	GFP-P2A-FLuc	$595		Add to Cart
BHV19400002-10	LTV-0002-10S	1x10^6	Blasticidin	GFP-P2A-FLuc	$595		Add to Cart
BHV19400002-11	LTV-0002-11S	1x10^6	Puromycin	RFP-P2A-FLuc	$595		Add to Cart
BHV19400002-12	LTV-0002-12S	1x10^6	Blasticidin	RFP-P2A-FLuc	$595		Add to Cart
BHV19400002-13	LTV-0002-13S	2x10^6	RFP (constitutively expressed)	GFP	$595		Add to Cart
BHV19400002-14	LTV-0002-14S	2x10^6	GFP (constitutively expressed)	RFP	$595		Add to Cart
BHV19400002-15	LTV-0002-15S	5x10^6	Puromycin	BFP	$595		Add to Cart
BHV19400002-16	LTV-0002-16S	5x10^6	Blasticidin	BFP	$595		Add to Cart
BHV19400002-17	LTV-0002-4SIC	2x10^6	Blasticidin	Renilla Luc	$595		Add to Cart

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