Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cells or experimental animals.This adenovirus expresses both Cre recombinase and GFP as marker. Cre and GFP are driven by 2 separate promoters. Check out this instruction video on using our adenovirus Cre to knockout LoxP flanked gene in primary mouse embryonic fibroblasts (MEF).
Categories
Adenovirus, Over-Expression, Recombinase
Product Type
Pre-Made
Virus Type
Adenovirus
Function
Reporter/Tag
Promoter
CMV (ubiquitous)
Reporter
eGFP
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)
Shelf Life
12 Months
HS Code
3822190000
UNSPSC Code
41106621
Insert Gene
Cre-GFP
Availability
Ready to Ship
Titer
1x10^10 PFU/ml
Reporter
eGFP
Storage
-80C
Shipping
Dry Ice
Reviews of Cre Recombinase Adenovirus (Ad-Cre-GFP)