codon-optimized FLP Recombinase (FLPo) Adenovirus (Ad-CMV-FLPo)

Mfr.No.	1775
Description	FLP from Saccharomyces cerevisiae, a site-specific recombinases (SSR) from the λ integrase family, recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency for both in vivo and in vitro in mouse. This adenovirus express FLPo under a control of a CMV promoter.
Categories	Adenovirus, Over-Expression, Recombinase
Product Type	Pre-Made
Virus Type	Adenovirus
Function	Recombinase
Promoter	CMV (ubiquitous)
Storage Buffer	DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone	Human Adenovirus Type5 (dE1/E3)
Shelf Life	12 Months
HS Code	3822190000
UNSPSC Code	41106621
Insert Gene	FLPo
Availability	Ready to Ship
Titer	1x10^10 PFU/ml
Storage	-80C
Shipping	Dry Ice

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SKU	Supplier No.	Size	Your price	Quantity
BHV21600343	1775	200 µL	$495		Add to Cart