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Home / ELISA Kit / Unconventional Sample Types ELISA

ELISA Kit for NEDD4 Binding Protein 2 (N4BP2) (Human)

SKU : BHE10108594

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CLOUD-CLONE CORP.(CCC)

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Mfr.No.	SES042Hu
Description	This assay has high sensitivity and excellent specificity for detection of NEDD4 Binding Protein 2 (N4BP2). No significant cross-reactivity or interference between NEDD4 Binding Protein 2 (N4BP2) and analogues was observed.
Alternative Names	B3BP; BCL3 Binding Protein
Reactivity	Human
Detection Method	Colormetric
Detection Range	0.312-20ng/mL
Sample Type	Other biological fluids, Tissue homogenates, Cell lysates
Assay Type	Sandwich
Assay Duration	3h
Kit Components	<table class="reagent_table"><tbody><tr class="first_tr"><td>Reagents</td><td>Quantity</td><td>Reagents</td><td>Quantity</td></tr><tr><td>Pre-coated, ready to use 96-well strip plate</td><td>1</td><td>Plate sealer for 96 wells</td><td>4</td></tr><tr><td>Standard</td><td>2</td><td>Standard Diluent</td><td>1×20mL</td></tr><tr><td>Detection Reagent A</td><td>1×120µL</td><td>Assay Diluent A</td><td>1×12mL</td></tr><tr><td>Detection Reagent B</td><td>1×120µL</td><td>Assay Diluent B</td><td>1×12mL</td></tr><tr><td>TMB Substrate</td><td>1×9mL</td><td>Stop Solution</td><td>1×6mL</td></tr><tr><td>Wash Buffer (30 × concentrate)</td><td>1×20mL</td><td>Instruction manual</td><td>1</td></tr> </tbody></table>
Features	Detects protein (regular version)
Species	Human
Sensitivity	0.117ng/mL
Precautions	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. <br />To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEDD4 Binding Protein 2 (N4BP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEDD4 Binding Protein 2 (N4BP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEDD4 Binding Protein 2 (N4BP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEDD4 Binding Protein 2 (N4BP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene ID	55728
UniProt ID	Q86UW6
Antigen	N4BP2
Usage Notes	1. Prepare all reagents, samples and standards;<br />2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;<br />3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;<br />4. Aspirate and wash 3 times;<br />5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;<br />6. Aspirate and wash 5 times;<br />7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;<br />8. Add 50µL Stop Solution. Read at 450nm immediately.

Applications	ELISA
Research Area	Signal transduction
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEDD4 Binding Protein 2 (N4BP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEDD4 Binding Protein 2 (N4BP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price	Quantity
BHE10108594	SES042Hu	96T*1	$770		Add to Cart
BHE10108594	SES042Hu	96T*5	$3465		Add to Cart
BHE10108594	SES042Hu	96T*10	$6545		Add to Cart

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