We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Tag
N-terminal 6xHis-tagged (This tag can be tested only under denaturing conditions)
Molecular Weight
29.1 kDa
Relevance
"Claudin 18 is a novel negative regulator of bone resorption and osteoclast differentiation." Linares G.R., Brommage R., Powell D.R., Xing W., Chen S.T., Alshbool F.Z., Lau K.H., Wergedal J.E., Mohan S. J Bone Miner Res 27:1553-1565(2012)
Source
Mammalian cell
Endotoxin Level
Less than 1.0 EU/ug as determined by LAL method.
Form
Lyophilized powder
Notes
Repeated freezing and thawing is not recommended. Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.
Bioactivity
Yes
Expression Region
1-264aa of Isoform A2.1
Lead Time
3-7 business days
Protein Length
Full Length of Isoform A2.1
Uniprot
P56857
Gene Name
Cldn18
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Purity
Testing in progress.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reviews of Recombinant Mouse Claudin-18.2 (Cldn18.2)-VLPs (Active)