We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Tag
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Molecular Weight
24.2 kDa
Relevance
"Claudin association with CD81 defines hepatitis C virus entry." Harris H.J., Davis C., Mullins J.G., Hu K., Goodall M., Farquhar M.J., Mee C.J., McCaffrey K., Young S., Drummer H., Balfe P., McKeating J.A. J. Biol. Chem. 285:21092-21102(2010)
Source
Mammalian cell
Endotoxin Level
Less than 1.0 EU/ug as determined by LAL method.
Form
Lyophilized powder
Notes
Repeated freezing and thawing is not recommended. Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.
Bioactivity
Yes
Expression Region
1-217aa
Lead Time
3-7 business days
Protein Length
Full Length
Uniprot
O95484
Gene Name
CLDN9
Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Purity
Testing in progress.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reviews of Recombinant Human Claudin-9 (CLDN9)-VLPs (Active)