N-terminal 10xHis-tagged and C-terminal Twin-Strep-tagged
Molecular Weight
27.7 kDa
Relevance
Plays a major role in tight junction-specific obliteration of the intercellular space.<br />(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
Source
Mammalian cell
Form
Liquid
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, pH7.5
Purity
Greater than 90% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. <br />Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reference
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."<br />Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Gray A.M.<br />Genome Res. 13:2265-2270 (2003)<br />"Complete sequencing and characterization of 21,243 full-length human cDNAs."<br />Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Sugano S.<br />Nat. Genet. 36:40-45 (2004)