5-HT3-A;5-HT3A;5-hydroxytryptamine receptor 3;5-HT-3;5-HT3R;Serotonin receptor 3A;Serotonin-gated ion channel receptor
Reconstitution
/
Tag
N-terminal 10xHis-Avi-tagged and C-terminal Twin-Strep-tagged
Molecular Weight
59.8 kDa
Relevance
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Source
Mammalian cell
Form
Liquid
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, pH7.5
Purity
Greater than 85% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. <br />Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.