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Home / Proteins & Peptide / Recombinant Protein

Recombinant Hepatitis B virus genotype C subtype adr Large envelope protein (S)-VLPs

SKU : BHP10515313

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-MP800387HEAA(F2)
Alternative Names	L glycoprotein;L-HBsAg;LHB;Large S protein;Large surface protein;Major surface antigen
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80°C.<br />Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
Tag	C-terminal 10xHis-tagged
Molecular Weight	32.6 kDa
Relevance	The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. ; The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.
Source	Mammalian cell
Form	Lyophilized powder
Notes	The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20°C/-80°C upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity.<br />The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Expression Region	1-281aa
Lead Time	18-28 business days
Protein Length	Full Length of Isoform M

Uniprot	Q81162-2
Gene Name	S
Research Area	Others
Sequence	MQWNSTTFHQALLDPRVRGLYFPAGGSSSGTVNPVPTIVSPISSIFSRTGDPAPNMESTTSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGEAPTCPGQNSQSPTSNHSPTSCPPICPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLLPGTSTTSTGPCKTCTIPAQGTSMFPSCCCTKPSDGNCTCIPIPSSWAFARFLWEWASVRFSWLSLLVPFVQWFAGLSPTVWLSVIWMMWYWGPSLYNILSPFLPLLPIFFCLWVYI

Buffer	Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4.
Purity	The purity information is not available for VLPs proteins.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. <br />Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Product Manual	<a href="https://www.cusabio.com/msds/12942550/" target="_blank">MSDS</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHP10515313	CSB-MP800387HEAA(F2)	20ug	$630		Add to Cart

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