(1) Q: Can serum be present when preparing nucleic acid transfection reagent complex?
A: The presence of serum will affect the formation of liposomes. It is recommended to use serum-free medium (generally MEM medium) when preparing nucleic acid transfection reagent complexes.
(2) Q: Can the transfection reagent be frozen?
A: This reagent must be stored at 2-8°C, and should be avoid repeated and prolonged opening of the cap, as long-term opening of the cap will cause liposome oxidation and affect the transfection efficiency.
(3) Q: What should I pay attention to when using Hieff Trans™ Universal Transfection Reagent?
A: 1) During the transfection operation, it is better that the cell confluence reaches 70%-90%, and the specific plating density is determined according to the situation of the cells.
2) Preparation of transfection complexes requires dilution of DNA and transfection reagents in serum-free medium.
3) Antibiotics cannot be added to the medium during transfection.
4) The use of high-purity DNA or RNA helps to obtain higher transfection efficiency, and endotoxin in plasmids is the enemy of transfection.
5) Store at 2-8ºC, be careful not to open the lid repeatedly for a long time.
6) The nucleic acid concentration and reagent volume should be optimized for the first use to obtain the highest transfection efficiency.
(4) Q: Does it need to be terminated after transfection?
A: No need. It is not necessary to remove the nucleic acid-transfection reagent complex or replace with fresh medium after transfection, and the fresh medium can also be replaced after 4-6 hours according to the nutrient status of the cells.
(5) Q: Can the transfection reagent be used for transfection of viral vector packaging?
A: Yes. The efficiency of viral vector packaging is not necessarily related to the efficiency of transfection, but also to the selection of packaging plasmids and the ratio between plasmids.
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