Hieff Trans^TM Ultra PEI AAV Transfection Reagent

Mfr.No.

40823ES03, 40823ES60, 40823ES10, 40823ES01

Description

This product is a novel linearized polyethyleneimine (Polyethylenimine Linear, PEI) specially developed for the large-scale production of AAV in suspension culture systems. It is a chemically synthesized product, free of animal-derived components, with low cytotoxicity. The product is provided in sterile liquid form and has a wide applicability for various AAV serotypes. It features strong DNA loading capacity, low transfection complex ratio, and high complex stability, facilitating large-scale AAV production.

Applications

Production of AAV Viral Vectors

Specifications

Form	liquid
Properties	Polyethylenimine Linear (PEI)
Virus Type	AAV

Components

Components No.	Name	40823ES01	40823ES03	40823ES10	40823ES60
40823	Hieff TransTM Ultra PEI-AAV Transfection Reagent	100 μL	1 mL	10 mL	100 mL

Features

Significant Increase in Virus Titer: The product achieves a substantial increase in virus yield, with approximately a 2-fold increase in virus titer for multiple serotypes, including AAV2, AAV5, AAV8, and AAV9.
Reduced Usage Cost: By reducing the amount of transfection reagent by 50% while maintaining high yield, the product effectively lowers production costs.

Storage	Hieff TransTM Ultra PEI-AAV Transfection Reagent products should be stored at 2-8℃ for 2 year.

Other Protocols	Safety Data Sheet Manual

AAV2, AAV5, AAV8, and AAV9 were produced in suspension 293F cells, with a DNA dosage of 1 µg per million cells. The virus was harvested 72 hours post-transfection, and the viral supernatant was analyzed.

AAV8 was produced in suspension 293F cells with DNA dosages of 0.5 µg, 0.75 µg, and 1 µg per million cells. The virus was harvested 72 hours post-transfection.

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Combination SKU	Supplier No.	Size	Your price
BHM20800012-1	40823ES03	1 mL	$365	Add to Cart
BHM20800012-2	40823ES60	100 mL	$8565	Add to Cart
BHM20800012-3	40823ES10	10 mL	$1745	Add to Cart
BHM20800012-4	40823ES01	100 uL	$225	Add to Cart

Hieff Trans^TM Ultra PEI AAV Transfection Reagent

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