0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
Host
Goat
Immunogen Region
Amino acids CPSRGQGKPS were used as the immunogen for this Sprouty antibody.
Isotype
Goat Ig
Species Reactivity
Human, Mouse
Note
Optimal dilution of the Sprouty antibody should be determined by the researcher.1. Reported by customer to detect Sprouty (nuclear staining) in FFPE mouse brain. 2. Reported by customer to detect Sprouty in embryonal rhabdomyosarcoma (ERMS) lysate.
Format
Antigen affinity purified
Purity
Antigen affinity
Storage
Aliquot and store the Sprouty antibody at -20oC.
Applications
ELISA (peptide), WB, IF/ICC, FACS, IHC-P
Description
Western blot: 1-3ug/ml (2),Immunohistochemistry (FFPE): 1-3ug/ml (1),Immunofluorescence/Immunocytochemistry: 10ug/ml,FACS: 10ug/million cells in 0.2ml,ELISA (peptide) LOD: 1:16000