Dylight 488, Goat Anti-Rabbit IgG-BHA13700290

Mfr.No.	A23220
Background	Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
Categories	Secondary Antibodies
Clonality	Polyclonal
Description	Dylight 488 conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody
Featured	DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).
Formulation	Liquid solution
Host	Goat
Immunogen	Rabbit IgG whole molecule
Immunogen Region	Rabbit IgG whole molecule
Isotype	Goat IgG
Others	The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity	Rabbit
Shipping	Gel pack with blue ice.
Tag	Goat Anti-Rabbit IgG
Note	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

Purification	Affinity purified using solid phase Rabbit IgG (H&L) with finally> 95% purity based on SDS-PAGE
Storage Buffer	Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Storage Instructions	Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Applications	ICC, IF, FCM
Dilution	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.