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Home / Secondary Antibodies

Alkaline Phosphatase Conjugated Affinity Purified anti-Sheep IgG [H&L] [Donkey]

BHA10907909
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Contact us to order
Tel
+1 866.986.9598
Email
orders@biohippo.com
Get a Quote
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Request More Infos
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Buffer 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Description Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Storage Condition -20C
Description This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Dilution

ELISA1:3,000-1:14,000
SKU Supplier No. Size Your price Quantity
BHA10907909 SA029 250 ug $217.50
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