mRNA Cap 2'-O-Methyltransferase GMP grade (50 U/ul)

Mfr.No.

10612ES97, 10612ES92

Description

mRNA Cap 2´-O-Methyltransferase is a recombinant protein derived from vaccinia virus. The enzyme can add a methyl group at the 2´-O position of the first nucleotide of the cap structure at the 5´ end of the RNA. The enzyme uses SAM as a methyl donor to methylate capped RNA to form a Cap1 structure. The Cap1 structure can enhance the translation efficiency of mRNA, so it can improve the expression of mRNA in transfection and microinjection experiments. This enzyme requires RNA with m7GpppN, a 7-methylguanosine cap structure, as a substrate.
This product is produced in accordance with GMP process requirements. The product is provided in liquid form and can be used for Cap1 capping reaction of pre-mRNA in vivo/in vitro.

Features

Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, Rnases

Kit Components

Components No.	Name	10612ES92 (10,000 U)	10612ES97 (50,000 U)	10612ES98 (250,000 U)	10612ES99 (20 MU)
10612	mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U/μl)	200 μL	1 mL	5 mL	400 mL

Specification

Source	Recombinant E.coli with Cap 2´-O-Methyltransferase gene
Optimum Temperature	37℃
Storage Buffer	20 mM Tris-HCl pH8.0, 0.1mM EDTA, 1mM DTT, 100mM NaCl, 50% (v/v) glycerin, 0.1% (v/v) Trion X-100
Unit Definition	One unit is defined as the amount of enzyme required to methylate 10 pmol of 80nt capped RNA transcript in 1 h at 37°C.

Shipping	The mRNA Cap 2´-O-Methyltransferase GMP-grade products are shipped with dry ice.
Storage	Stored at -15℃ ~ -25℃ for one year.

Product Manual	Safety Data Sheet MSDS-10612-mRNA Cap 2´-O-Methyltransferase GMP-grade (50 U_μl)-HB220701.pdf Manuals 10612_Manual_HB221110_EN.pdf

Application	2´-O-methylation of capped in vitro transcripts helps the RNA evade innate immune response in vivo mRNA cap-1 structure may improve expression

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHN20800034-1	10612ES97	50000 U	$685		Add to Cart
BHN20800034-2	10612ES92	10000 U	$165		Add to Cart

mRNA Cap 2'-O-Methyltransferase GMP grade (50 U/ul)

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