LZCap GG (3'Acm), (100mM)

Mfr.No.

10686ES60, 10686ES80

Description

LZCap GG (3'Acm) is a cap1 analog. The product is used for transcription with the initial sequence of 5'GG3', and the natural cap1 structure is produced by Cap1 transcription capping.

Kit Components

Component	Volume (μL)	Final concentration
RNase Free Water	Up to 20μL	/
ATP(100mM)	1	5mM
UTP(100mM)	1	5mM
CTP(100mM)	1	5mM
GTP(100mM)	0.2	1mM
LZCap®GG(3'Acm) (100mM)	0.8	4mM
10×Transcription Buffer	2	1 ×
Linear DNA	1μg	50 ng/μL
SP6 Enzyme Mix	2	/
Final Volume	20μL

Notes

1) The SP6 enzyme mix we recommended to used is MEGAscript® Kit（AM1330）.

2) LZCap®GG(3'Acm) is suitable for SP6 promoter transcription vector with 5 ‘GG 3’initiated sequences, which needs to be considered when constructing the vector.

3) The reagents, consumables and containers used in the experiment are free of RNase contamination.

4) It is recommended to use a linearized DNA template for transcription.

5) When modified nucleotides were used in place of wild-type nucleotides, the final concentration of the reaction was unchanged.

6) Modified N1-Me-pUTP can be used in place of wild-type UTP. The modified N1-Me-pUTP reduces the immunogenicity of mRNA. Henovcom can also provide modified nucleotide N1-Me-pUTP (Cat. No.: HN1002).

7) If the PCR product is used as the transcription initiation DNA template, the amount of DNA template can be reduced by half.

Molecular Formula

C₃₅H₄₈N₁₆O₂₅P₄ (Free acid)

Precautions

For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
For research use only.

Concentration

100± 3 mM

Molecular Weight

1216.19 (Free acid)

Purity	HPLC ≥95%
Storage	This product could be stored at -25~-15 ℃ for two years.

Protocol	LZCap® DNA Template Design LZCap®GG(3'Acm) is suitable for GG-initiated sequences. As shown in the figure below, the SP6 promoter (underlined) followed by the GG sequence can effectively initiate transcription. Protocol 1． Thaw components required for the experiment on ice. 2． Add RNase free water and NTPs to reaction tube. 3． Heat LZCap®GG(3'Acm) aliquot for 15 min at 60°C. Cool at room temperature for 5 min. 4． Immediately add LZCap®GG(3'Acm) to tube and vortex to mix. Spin briefly to collect liquid. 5． Add 10x Transcription Buffer and vortex. 6． Add DNA template and SP6 Enzyme Mix. 7． Mix the prepared reaction solution, spin briefly, and incubate at 37°C for 2-3 hours. If the transcript length is less than 100nt, increase the reaction time to 4-8 h.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHN20800031-1	10686ES60	100 uL	$1000		Add to Cart
BHN20800031-2	10686ES80	1 mL	$8000		Add to Cart

LZCap GG (3'Acm), (100mM)

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