Recombinant Human Dynamin-1-like protein (DNM1L)

Mfr.No.	CSB-EP007078HU
Relevance	Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution.
Source	E.coli
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Protein Name	Dnm1p/Vps1p-like protein (DVLP) (Dynamin family member proline-rich carboxyl-terminal domain less; ) (Dymple) (Dynamin-like protein) (Dynamin-like protein 4) (Dynamin-like protein IV) (HdynIV) (Dynamin-related protein 1) (DLP1) (DRP1)
Expression Region	1-710aa
Mol Weight	84.4 kDa
Protein Description	Full Length of Isoform 2

Involvement In Disease	Encephalopathy due to defective mitochondrial and peroxisomal fission 1 (EMPF1)
Subcellular Location	Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Peripheral membrane protein, Mitochondrion outer membrane, Peripheral membrane protein, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane
Uniprot	O00429
Database Links	HGNC:2973 OMIM:603850 KEGG:hsa:10059 STRING:9606.ENSP00000450399 UniGene:Hs.556296
Function	Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis
Pathway	TNF signaling pathway; Necroptosis; NOD-like receptor signaling pathway
Protein Families	TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
Research Area	Cancer
Tissue Specificity	Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in b

Purity	Greater than 85% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Storage Buffer	Tris-based buffer，50% glycerol