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Home / Primary Antibodies / Recombinant Antibodies

Ubiquitin Antibody, Clone RGL3R (SMC-548)

BHA11906072
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Antigen Ubiquitin
Swissprot P0CG47/P0CG48
Gene Id 7314
Target Ubiquitin
Clone No RGL3R
Research Area Cell Signaling, Post-translational Modifications, Ubiquitination, Neurodegeneration, Alzheimer's Disease, Neuroscience
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 1 mg/ml
Dilutions WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkappaBalpha and p27 (5).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Trademark RECOMBINANT
Applications ELISA, WB
Description WB ; ELISA
Dilution WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189.

2. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57.

3. Boston Biochem: "Ubiquitin Proteasome Pathway Overview” http://www.bostonbiochem.com/upp.php

4. Yang M., et al. (1998) J Exp Med. 187: 1835-1846.

5. Chen Z.J., et al. (1996) Cell 84: 853-862.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906072-1 SMC-548D Unconjugated 100 ug $384
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BHA11906072-2 SMC-548D-A390 ATTO 390 100 ug $439
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BHA11906072-3 SMC-548D-A488 ATTO 488 100 ug $438
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BHA11906072-4 SMC-548D-A594 ATTO 594 100 ug $438
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BHA11906072-5 SMC-548D-APC APC 100 ug $437
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BHA11906072-6 SMC-548D-BI Biotin 100 ug $434
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BHA11906072-7 SMC-548D-FITC FITC 100 ug $427
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BHA11906072-8 SMC-548D-HRP HRP 100 ug $423
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BHA11906072-9 SMC-548D-PCP PerCP 100 ug $437
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BHA11906072-10 SMC-548D-RPE RPE 100 ug $435
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BHA11906072-11 SMC-548S Unconjugated 12 ug $45
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