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Home / Primary Antibodies / Recombinant Antibodies

HSP70 Antibody, Clone 1H11 (SMC-249)

BHA11902056
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Antigen HSP70
Swissprot P0DMV8/P0DMV9
Accession Number NP_005336.3
Gene Id 3303
Target HSP70
Clone No 1H11
Research Area Cell Signaling, Chaperone Proteins, Cancer, Tumor Biomarkers, Heat Shock, Protein Trafficking
Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration 0.5 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100), FACS (1:250); optimal dilutions for assays should be determined by the user.
Purification Protein G Purified
Purity Induced by heat shock.
Scientific Background HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6).
Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9).
Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.
Shipping Temperature Blue Ice or 4°C
Specificity Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa band in Western Blot.
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications FACS, WB, ICC/IF, FCM
Description WB ; ICC ; IF ; FCM ; FACS
Dilution WB (1:1000), ICC/IF (1:100), FACS (1:250); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Gribaldo, S. et al. (1999) J. Bacteriol. 181, 434-443.

2. Sharma, D. & Masison, D.C. (2009) Protein Pept. Lett. 16, 571-581.

3. Sharma, D. et al. (2009) PLoS. ONE.4, e6644.

4. Kampinga, H.H. & Craig, E.A. (2010) Nat. Rev. Mol. Cell Biol. 11, 579-592.

5. Nickel, W. & Seedorf, M. (2008) Annu. Rev. Cell Dev. Biol. 24, 287-308.

6. Multhoff, G. & Hightower, L.E. (1996) Cell Stress. Chaperones. 1, 167-176.

7. De Maio, A. (2011) Cell Stress. Chaperones. 16, 235-249.

8. Horvath, I. & Vigh, L. (2010) Nature. 463, 436-438.

9. Horvath, I., Multhoff, G., Sonnleitner, A., & Vigh, L. (2008) Biochim. Biophys. Acta 1778, 1653-1664.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11902056-1 SMC-249D Unconjugated 100 ug $401
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BHA11902056-2 SMC-249D-A390 ATTO 390 100 ug $456
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BHA11902056-3 SMC-249D-A488 ATTO 488 100 ug $455
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BHA11902056-4 SMC-249D-A594 ATTO 594 100 ug $455
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BHA11902056-5 SMC-249D-APC APC 100 ug $453
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BHA11902056-6 SMC-249D-BI Biotin 100 ug $450
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BHA11902056-7 SMC-249D-FITC FITC 100 ug $446
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BHA11902056-8 SMC-249D-HRP HRP 100 ug $441
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BHA11902056-9 SMC-249D-PCP PerCP 100 ug $453
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BHA11902056-10 SMC-249D-RPE RPE 100 ug $451
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BHA11902056-11 SMC-249S Unconjugated 12 ug $45
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