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Home / Primary Antibodies / Monoclonal Antibodies

Recombinant AMACR Antibody / p504S

SKU : BHA17116348

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NSJ Bioreagents

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Mfr.No.	V7480, V7480-20UG, V7480-100UG
Clonality	Monoclonal
Description	This antibody recognizes a protein of 43kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Rabbit
Immunogen Region	A portion of amino acids 297-394 was used as the immunogen for the recombinant AMACR antibody.
Isotype	IgG,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	Yes
Subcellular Location	Cytoplasmic

Antigen	AMACR / p504S
Uniprot	Q9UHK6
Clone No	AMACR/2748R

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein A affinity chromatography
Storage	Store the recombinant AMACR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	IHC-P, WB
Dilution	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml

Product Manual	Data Sheet

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