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Home / Biochemicals / Reagents

Ready-to-load DPPC Liposomes with Ammonium Sulfate

SKU : BHE16300409

This product includes 10 ml liposomes with 10 mg/ml lipid concentration. The mass ratio of lipids is DPPC: cholesterol: PEGylated DSPE = 1.9:1. The concentration of ammonium sulfate encapsulated within the liposomes for drug loading is 200 mM. The buffer is 10 mM histidine, pH 6.5, 9.2 % sucrose. Liposomes are stored in a 2°C to 8°C refrigerator. DO NOT freeze liposomes.

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Mfr.No.	DPC100AS
Description	Liposomal drug formulations provide great opportunities of improving the drug efficacy and toxicity profiles. Drug molecules with amine groups such as doxorubicin, daunorubicin, topotecan and irinotecan etc. can be loaded by ammonium-based pH gradient. The Ready-to-load DPPC Liposomes with Ammonium Sulfate (Catalog number DPC100AS) are high quality non-PEGylated liposomes that are ready to load drug molecules containing amine groups. The drug loading process is completed in about 3 hours after mixing the drug with the liposomes. The composition of the liposomes is 1,2-dipalmitoyl-sn-glycero-3-phophocholine (DPPC) and cholesterol in the weight ratio of 1.9:1. The total lipid concentration is 10 mg/ml. The liposomes are encapsulated with ammonium sulfate for drug remote loading. The average size of liposomes is about 90 nm with poly dispersity index (dpi) of 0.1. The Ready-to-load DPPC Liposomes with Ammonium Sulfate (Catalog number DPC100AS) includes 10 ml liposomes with 10 mg/ml lipid concentration. The mass ratio of lipids is DPPC: cholesterol = 1.9:1. The concentration of ammonium sulfate encapsulated within the liposomes for drug loading is 200 mM. The buffer is 10 mM histidine, pH 6.5, 9.2 % sucrose. Liposomes are stored in a 2°C to 8°C refrigerator. DO NOT freeze liposomes.
Liposomes	Liposomes for loading

Shipping Temperature	Cold but not frozen

Product Manual	Datasheet
Protocol	DRUG LOADING PROTOCOL Drug molecules containing at least one amine group are potentially loaded into the liposomes. The drug loading capacity depends on the property of the drug molecules. The typical drug to lipids ratio is from 1:5 to 1:20. 1. Drug loading Mix the drug solution and the liposome and incubate the mixture in a 48°C water bather for 3 hours. Save the loaded liposomes at 2 to 8°C. 2. Drug encapsulation measurement The yield of drug encapsulation is measured by a spin-column method using the Liposome Drug Encapsulation Assay kit (Catalog number LDE10). A volume of 50 µl of sample was loaded on the spin column and eluted with 100 µl of the elution buffer. The encapsulated dug is eluted and the non-encapsulated drug stays on the column. For detailed protocol, please see the Instruction of the Liposome Drug Encapsulation Assay kit (Catalog number LDE10).

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300409	DPC100AS	10 ml	$255.88		Add to Cart

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