Bovine Growth Hormone (GH) (Competitive EIA) ELISA Kit

Mfr.No.

CEA044Bo

Sensitivity

50.2pg/mL

Detection Range

123.5-10,000pg/mL

Reactivity

Bovine

Assay Duration

Assay Type

Competitive Inhibition

Description

This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH).
No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.

Alternative Names

GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Hormone (GH) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Hormone (GH) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	97-104	101
EDTA plasma(n=5)	78-101	91
heparin plasma(n=5)	99-105	102

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Hormone (GH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	83-92%	96-105%	95-105%	79-90%
EDTA plasma(n=5)	79-93%	81-105%	89-99%	81-99%
heparin plasma(n=5)	91-105%	82-102%	80-96%	87-94%

Features

Detects small molecule

Species

Bovine

Sample Type

Cell lysates, Cell culture supernates, Serum, Tissue homogenates, Plasma, Other biological fluids

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Growth Hormone (GH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Growth Hormone (GH) and unlabeled Growth Hormone (GH) (Standards or samples) with the pre-coated antibody specific to Growth Hormone (GH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Growth Hormone (GH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Growth Hormone (GH) in the sample.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Gene ID	280804
UniProt ID	P01246

Applications	ELISA
Research Area	Endocrinology

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

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SKU	Supplier No.	Size	Your price
BHE10100310	CEA044Bo	96T*1	$718	Add to Cart
BHE10100310	CEA044Bo	96T*5	$3231	Add to Cart
BHE10100310	CEA044Bo	96T*10	$6103	Add to Cart

ELISA Kit for Growth Hormone (GH) (Bovine)

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