NHE3

Mfr.No.	SPC-400, SPC-400D, SPC-400D-A390, SPC-400D-A488, SPC-400D-A594, SPC-400D-APC, SPC-400D-BI, SPC-400D-FITC, SPC-400D-HRP, SPC-400D-PCP, SPC-400D-RPE, SPC-400S
Immunogen Species	Rat
Alternative Names	Sodium / Hydrogen Exchanger 3 antibody, SLC9A3 Antibody, Na+/H+ exchanger 3 Antibody, isoform 3 Antibody, Solute carrier family 9 member 3 Antibody, NHE3MGC126720 Antibody, MGC126718 Antibody, solute carrier family 9 (sodium/hydrogen exchanger) member 3 Antibody, NHE-3 Antibody, Na(+)/H(+) exchanger 3 Antibody, Sodium/hydrogen exchanger 3 Antibody
Categories	Antibodies
Cellular Localization	Membrane
Certificate Of Analysis	1 ug/ml of SPC-400 was sufficient for detection of HNE3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Rat NHE3 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide mapping to AA 809 to 831 of rat sequence, Rattus Norvegicus (Rat)
Immunogen Region	Synthetic peptide mapping to AA 809 to 831 of rat sequence
Launch Date	2010/06/11
Net Weight (g)	0.1
Reactivity	Rat, Mouse
Recombinant	FALSE
Regulatory	RUO
Species	Rattus Norvegicus (Rat)
Species Reactivity Medium	Rat, Mouse
Tissue Specificity	Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level)

Antigen	NHE3
Swissprot	P26433
Accession Number	NP_036786
Gene Id	24784
Target	NHE3
Research Area	Cell Signaling, Pumps/Transporters, Cancer, Ion Pumps/Transporters, Neuroscience

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Purity	Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level)
Scientific Background	Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~84kDa.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942. 2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11907748-1	SPC-400D	Unconjugated	100 ug	$348	Add to Cart
BHA11907748-2	SPC-400D-A390	ATTO 390	100 ug	$403	Add to Cart
BHA11907748-3	SPC-400D-A488	ATTO 488	100 ug	$402	Add to Cart
BHA11907748-4	SPC-400D-A594	ATTO 594	100 ug	$402	Add to Cart
BHA11907748-5	SPC-400D-APC	APC	100 ug	$401	Add to Cart
BHA11907748-6	SPC-400D-BI	Biotin	100 ug	$397	Add to Cart
BHA11907748-7	SPC-400D-FITC	FITC	100 ug	$393	Add to Cart
BHA11907748-8	SPC-400D-HRP	HRP	100 ug	$389	Add to Cart
BHA11907748-9	SPC-400D-PCP	PerCP	100 ug	$401	Add to Cart
BHA11907748-10	SPC-400D-RPE	RPE	100 ug	$399	Add to Cart
BHA11907748-11	SPC-400S	Unconjugated	12 ug	$45	Add to Cart

Rabbit Anti-Rat NHE3 Polyclonal (SPC-400)