Erk1/2

Mfr.No.	SPC-120, SPC-120D, SPC-120D-A390, SPC-120D-A488, SPC-120D-A594, SPC-120D-APC, SPC-120D-BI, SPC-120D-FITC, SPC-120D-HRP, SPC-120D-PCP, SPC-120D-RPE, SPC-120S
Immunogen Species	Rat
Alternative Names	ERK1 Antibody, ERK2 Antibody, ERT1 Antibody, ERT2 Antibody, MAP kinase1 Antibody, MAP kinase2 Antibody, MAPK1 Antibody, MAPK2 Antibody, MAPK3 Antibody, p38 Antibody, p40 Antibody, p41 Antibody, p41mapk Antibody, p42 MAPK Antibody, p44 ERK1 Antibody, p44 MAPK Antibody, PRKM1 PRKM2 Antibody, PRKM3 Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Nucleus
Certificate Of Analysis	A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
Clonality	Polyclonal
Description	Rabbit Anti-Rat Erk1/2 Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH., Rattus Norvegicus (Rat)
Immunogen Region	A 35 residue synthetic peptide, corresponding to Rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Launch Date	2008/05/01
Net Weight (g)	0.1
Reactivity	Fruit Fly, African clawed frog, Rat, Sheep, Mouse, Human, Bovine, Chicken
Recombinant	FALSE
Regulatory	RUO
Species	Rattus Norvegicus (Rat)
Species Reactivity Medium	Mouse, Rat, Bovine, Sheep, Chicken, Drosophila, Xenopus, Human

Antigen	ERK1
Swissprot	P21708
Accession Number	NP_059043.1
Gene Id	50689
Target	ERK1
Research Area	Cell Signaling, Post-translational Modifications, Phosphorylation, Neurodegeneration, Alzheimer's Disease, Neuroscience

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~44kda (ERK1) and ~42kDa (ERK2).
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB, ICC/IF, FCM
Description	WB ; IHC ; ICC ; IF ; FCM
Dilution	WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Boulton TG. et al. (1991) Biochemistry. 30(1):278-86. 2. Yoon S., and Seger R. (2006) Growth Factors 24:21-44. 3. Wolf G. (2005) Antioxid Redox Signal 7:1337-1345. 4. Chuerland D., Marmor G., Shainskaya A. and Seger R. (2008) J Biol Chem. Epub: http://www.jbc.org/cgi/doi/10.1074/jbc.M709030200 5. Zhuang S., and Schnellmann R.G. (2006) J Pharmacol Exp Ther 319:991-997.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11906563-1	SPC-120D	Unconjugated	100 ul	$288	Add to Cart
BHA11906563-2	SPC-120D-A390	ATTO 390	100 ul	$343	Add to Cart
BHA11906563-3	SPC-120D-A488	ATTO 488	100 ul	$341	Add to Cart
BHA11906563-4	SPC-120D-A594	ATTO 594	100 ul	$341	Add to Cart
BHA11906563-5	SPC-120D-APC	APC	100 ul	$340	Add to Cart
BHA11906563-6	SPC-120D-BI	Biotin	100 ul	$337	Add to Cart
BHA11906563-7	SPC-120D-FITC	FITC	100 ul	$333	Add to Cart
BHA11906563-8	SPC-120D-HRP	HRP	100 ul	$327	Add to Cart
BHA11906563-9	SPC-120D-PCP	PerCP	100 ul	$340	Add to Cart
BHA11906563-10	SPC-120D-RPE	RPE	100 ul	$338	Add to Cart
BHA11906563-11	SPC-120S	Unconjugated	12 ul	$45	Add to Cart

Rabbit Anti-Rat Erk1/2 Polyclonal (SPC-120)

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